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Cytotoxic Sesquiterpenes from Sonchus oleraceus.
Artigo em Inglês | MEDLINE | ID: mdl-31894000


Sonchus oleraceus is a large and widespread plant in the world. It is edible to humans as a leaf vegetable and also a folklore medicinal herb in the treatment of infections and inflammatory disease, but limited research on the chemical constituents of was presented.


To isolate and identified the bioactive ingredients from S. oleraceus.


20kg of S. oleraceus was extracted twice with 75% alcohol. The concentrated extract was suspended in H2O and partitioned with petroleum ether, dichloromethane, ethyl acetate and n-butanol, respectively. The ethyl acetate phase was subjected to repeated normal chromatography on a silica gel column chromatography and eluted with a gradient of CH2Cl2-MeOH to give 12 crude fractions. Fraction 6 was subjected to ODS silica gel column chromatography, Sephadex LH-20 and HPLC to yield 1 and 2. Cell viability of 1 and 2 on A549, H292 and Caco2 cell lines were assayed by MTT method. Apoptosis analysis and apoptosis related proteins were detected subsequently.


Two new sesquiterpenes were isolated from S. oleraceus and identified by NMR spectra and HR-ESI-MS. 1 selectively suppressed the viability of A549 and H292 cells with IC50 values of 14.2, and 19.5µM respectively, while possessing no cytotoxicity against Caco2 cells (IC50 > 100µM). 2 did not exhibit cytotoxicity against A549, H292 and Caco2 cells (IC50 > 100µM). 1 significantly decreased the density of live cells and could cause cell apoptosis at 10 and 20µM in a dose-dependent manner. After treatment of 1 for 24h, the level of cleaved caspase-3 was increased accompanied with the reduction in procaspase-3 expression, and the down-regulation of Bcl-2 was associated with the enhancement of Bax expression. 1 could led to the up-regulation of cytochrome c and activation of caspase-9.


1 and 2 are new sesquiterpenes from S. oleraceus. 1 could induce apoptosis in A549 and H292 cells through Bax/caspase-9 pathway.





Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Assunto da revista: Antineoplasicos / Química Ano de publicação: 2020 Tipo de documento: Artigo