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Tubulin Folding Cofactor TBCB is a Target of the Salmonella Effector Protein SseK1.
Araujo-Garrido, Juan Luis; Baisón-Olmo, Fernando; Bernal-Bayard, Joaquín; Romero, Francisco; Ramos-Morales, Francisco.
Afiliação
  • Araujo-Garrido JL; Departamento de Genética, Facultad de Biología, Universidad de Sevilla, 41012 Sevilla, Spain.
  • Baisón-Olmo F; Departamento de Genética, Facultad de Biología, Universidad de Sevilla, 41012 Sevilla, Spain.
  • Bernal-Bayard J; Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile.
  • Romero F; Departamento de Genética, Facultad de Biología, Universidad de Sevilla, 41012 Sevilla, Spain.
  • Ramos-Morales F; Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, 41012 Sevilla, Spain.
Int J Mol Sci ; 21(9)2020 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-32366039
ABSTRACT
Salmonella enterica serovar Typhimurium is a human and animal pathogen that uses type III secretion system effectors to manipulate the host cell and fulfill infection. SseK1 is a Salmonella effector with glycosyltransferase activity. We carried out a yeast two-hybrid screen and have identified tubulin-binding cofactor B (TBCB) as a new binding partner for this effector. SseK1 catalyzed the addition of N-acetylglucosamine to arginine on TBCB, and its expression promoted the stabilization of the microtubule cytoskeleton of HEK293T cells. The conserved Asp-x-Asp (DxD) motif that is essential for the activity of SseK1 was required for the binding and modification of TBCB and for the effect on the cytoskeleton. Our study has identified a novel target for SseK1 and suggests that this effector may have a role in the manipulation of the host cell microtubule network to provide a safe niche for this pathogen.

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Ano de publicação: 2020 Tipo de documento: Artigo País de afiliação: Espanha