Hepatitis E virus is effectively inactivated in platelet concentrates by ultraviolet C light.
Vox Sang
; 115(7): 555-561, 2020 Oct.
Article
em En
| MEDLINE
| ID: mdl-32383163
ABSTRACT
BACKGROUND AND OBJECTIVES:
As previous investigations have shown, THERAFLEX UV-Platelets, a UVC-based pathogen inactivation (PI) system, is effective against non-enveloped transfusion-relevant viruses such as hepatitis A virus (HAV), which are insensitive to most PI treatments for blood products. This study investigated the PI efficacy of THERAFLEX UV-Platelets against HEV in platelet concentrates (PCs). MATERIALS ANDMETHODS:
Buffy coat-derived PCs in additive solution were spiked with cell culture-derived HEV and treated with the THERAFLEX UV-Platelets system using various doses of UVC (0·05, 0·10, 0·15 and 0·20 (standard) J/cm2 ). Titres of infectious virus in pre- and post-treatment samples were determined using a large-volume plating assay to improve the detection limit of the virus assay.RESULTS:
THERAFLEX UV-Platelets dose-dependently inactivated HEV in PCs. The standard UVC dose inactivated the virus to below the limit of detection, corresponding to a mean log reduction of greater than 3·5.CONCLUSION:
Our study demonstrates that the THERAFLEX UV-Platelets system effectively inactivates HEV in PCs.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Raios Ultravioleta
/
Plaquetas
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Vírus da Hepatite E
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Segurança do Sangue
Limite:
Humans
Idioma:
En
Revista:
Vox Sang
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Alemanha
País de publicação:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
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INGLATERRA
/
REINO UNIDO
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SCOTLAND
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UK
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UNITED KINGDOM