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Dioscin exhibits anti-inflammatory effects in IL-1ß-stimulated human osteoarthritis chondrocytes by activating LXRα.
Wang, Haitao; Zhu, Haifeng; Yang, Xiaodong.
Afiliação
  • Wang H; Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Zhejiang, China.
  • Zhu H; Key Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province, Zhejiang, China.
  • Yang X; Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Zhejiang, China.
Immunopharmacol Immunotoxicol ; 42(4): 340-345, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32515238
ABSTRACT

OBJECTIVE:

Osteoarthritis (OA) is the most common joint disease that characterized by the degradation of articular cartilage. In this study, we aimed to investigate the anti-inflammatory activity of dioscin on IL-1ß-stimulated human osteoarthritis chondrocytes.

METHODS:

The production of PGE2 and NO was measured in this study. MMP1 and MMP3 were detected by ELISA. The expression of LXRα and NF-κB were tested by western blot analysis.

RESULTS:

Treatment of dioscin suppressed the production of PGE2 and NO, as well as the expression of COX-2 and iNOS (their key regulatory genes). Dioscin also attenuated the secretion of MMP1 and MMP3. Furthermore, dioscin inhibited the phosphorylation of NF-κB p65 and IκBα induced by IL-1ß. The degradation of IκBα induced by IL-1ß was also suppressed by dioscin. Dioscin increased the expression of LXRα and pretreatment of GGPP, the LXRα inhibitor, blocked the anti-inflammatory effects of dioscin.

CONCLUSIONS:

In conclusion, this study indicated that dioscin-mediated anti-inflammatory effect may be involved in the activation of LXRα.
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Immunopharmacol Immunotoxicol Assunto da revista: Alergia e Imunologia / Farmacologia / Toxicologia Ano de publicação: 2020 Tipo de documento: Artigo País de afiliação: China

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Immunopharmacol Immunotoxicol Assunto da revista: Alergia e Imunologia / Farmacologia / Toxicologia Ano de publicação: 2020 Tipo de documento: Artigo País de afiliação: China
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