Your browser doesn't support javascript.
loading
Endothelial Cells: Co-culture Spheroids.
Kanczler, Janos M; Wells, Julia A; Oreffo, Richard O C.
Afiliação
  • Kanczler JM; Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Faculty of Medicine, University of Southampton, Southampton, UK.
  • Wells JA; Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Faculty of Medicine, University of Southampton, Southampton, UK.
  • Oreffo ROC; Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Faculty of Medicine, University of Southampton, Southampton, UK. roco@soton.ac.uk.
Methods Mol Biol ; 2206: 47-56, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32754810
ABSTRACT
The development and maintenance of a functioning vascular system is a critical function for many aspects of tissue growth and regeneration. Vascular endothelial cell in vitro co-culture spheroids are self-organized cell composites that have the capacity to recapitulate the three-dimensional tissue microenvironment. These spheroid testing platforms aim to better understand the mechanisms of functional tissue and how new therapeutic agents can drive these 3D co-culture processes. Here we describe direct cell-cell 3D endothelial co-culture spheroid methods, to examine the physiological spatial growth and cell-cell interaction of vascular cells and surrounding native tissue cells in the formation of vascular networks within spheroids and the potential to regenerate tissue.
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Methods Mol Biol Assunto da revista: Biologia Molecular Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: Reino Unido

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Methods Mol Biol Assunto da revista: Biologia Molecular Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: Reino Unido