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High-Resolution Confocal Imaging of Pericytes in Human Fetal Brain Microvessels.
Errede, Mariella; Girolamo, Francesco; Virgintino, Daniela.
Afiliação
  • Errede M; Human Anatomy and Histology Unit, Department of Basic Medical Sciences, Neurosciences and Sensory Organs, Bari University School of Medicine, Bari, Italy. mariella.errede@uniba.it.
  • Girolamo F; Human Anatomy and Histology Unit, Department of Basic Medical Sciences, Neurosciences and Sensory Organs, Bari University School of Medicine, Bari, Italy.
  • Virgintino D; Human Anatomy and Histology Unit, Department of Basic Medical Sciences, Neurosciences and Sensory Organs, Bari University School of Medicine, Bari, Italy.
Methods Mol Biol ; 2206: 143-150, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32754816
ABSTRACT
Pericytes are integral part of neurovascular unit and play a role in the maintenance of blood-brain barrier integrity, angiogenesis, and cerebral blood flow regulation. Despite their important functional roles, a univocal phenotypic identification is still emerging also for the lack of a "pan-pericyte" marker. In the present study, we describe in detail the method for performing fluorescence immunohistochemistry on thick free-floating sections from human fetal brain in high resolution laser confocal microscopy. This method enables to obtain three-dimensional images of pericytes and provides insights about their distribution and localization in the microvessels of human developing brain.
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Methods Mol Biol Assunto da revista: Biologia Molecular Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: Itália

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Methods Mol Biol Assunto da revista: Biologia Molecular Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: Itália