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In Vivo Vascular Network Forming Assay.
Kim, Hwan D; Lin, Ruei-Zeng; Melero-Martin, Juan M.
Afiliação
  • Kim HD; Department of Cardiac Surgery, Boston Children's Hospital, Boston, MA, USA.
  • Lin RZ; Department of Surgery, Harvard Medical School, Boston, MA, USA.
  • Melero-Martin JM; Department of Cardiac Surgery, Boston Children's Hospital, Boston, MA, USA.
Methods Mol Biol ; 2206: 193-203, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32754819
ABSTRACT
The capability of forming functional blood vessel networks is critical for the characterization of endothelial cells. In this chapter, we will review a modified in vivo vascular network forming assay by replacing traditional mouse tumor-derived Matrigel with a well-defined collagen-fibrin hydrogel. The assay is reliable and does not require special equipment, surgical procedure, or a skilled person to perform. Moreover, investigators can modify this method on-demand for testing different cell sources, perturbation of gene functions, growth factors, and pharmaceutical molecules, and for the development and investigation of strategies to enhance neovascularization of engineered human tissues and organs.
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Methods Mol Biol Assunto da revista: Biologia Molecular Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: Estados Unidos

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Methods Mol Biol Assunto da revista: Biologia Molecular Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: Estados Unidos