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Effects of Acanthopanax senticosus (Rupr. & Maxim.) Harms on cerebral ischemia-reperfusion injury revealed by metabolomics and transcriptomics.
Chen, Ren-Hao; Du, Wei-Dong; Wang, Qi; Li, Zhi-Feng; Wang, Dong-Xu; Yang, Shi-Lin; Feng, Yu-Lin.
Afiliação
  • Chen RH; Jiangxi University of Traditional Chinese Medicine, Nanchang, 330002, China.
  • Du WD; Jiangxi University of Traditional Chinese Medicine, Nanchang, 330002, China.
  • Wang Q; State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang, 330006, China.
  • Li ZF; Jiangxi University of Traditional Chinese Medicine, Nanchang, 330002, China; Nanchang Key Laboratory of Active Ingredients of Traditional Chinese Medicine and Natural Medicine, Nanchang, 330006, China. Electronic address: 20101040@jxutcm.edu.cn.
  • Wang DX; Jiangxi University of Traditional Chinese Medicine, Nanchang, 330002, China.
  • Yang SL; State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang, 330006, China.
  • Feng YL; State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang, 330006, China. Electronic address: 20081002@jxutcm.edu.cn.
J Ethnopharmacol ; 264: 113212, 2021 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-32768643
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE Cerebral ischemia-reperfusion (CIR) injury is one of the main diseases leading to death and disability. Acanthopanax senticosus (Rupr. & Maxim.) Harms (AS), also known as Panax ginseng, has neuroprotective effects on anti-CIR injury. However, the underlying molecular mechanism of its therapeutic effects is not clear. AIM OF THE STUDY To systematically study and explore the mechanism of Acanthopanax senticosus (Rupr. & Maxim.) Harms extract (ASE) in the treatment of CIR injury based on metabolomics and transcriptomics. MATERIALS AND

METHODS:

The pharmacological basis of ASE in the treatment of CIR was evaluated, and samples were used in plasma metabolomics and brain tissue transcriptomics to reveal potential biomarkers. Finally, according to online database, we analyzed biomarkers identified by the two technologies, explained reasons for the therapeutic effect of ASE, and identify therapeutic targets.

RESULTS:

A total of 53 differential metabolites (DMs) were identified in plasma and 3138 differentially expressed genes (DEGs) were identified in brain tissue from three groups of rats, including sham, ischemia-reperfusion (I/R), and ASE groups. Enrichment analysis showed that Nme6, Tk1, and Pold1 that are involved in the production of deoxycytidine and thymine were significantly up-regulated and Dck was significantly down-regulated by the intervention with ASE. These findings indicated that ASE participates in the pyrimidine metabolism by significantly regulating the balance between dCTP and dTTP. In addition, ASE repaired and promoted the lipid metabolism in rats, which might be due to the significant expression of Dgkz, Chat, and Gpcpd1.

CONCLUSIONS:

The findings of this study suggest that ASE regulates the significant changes in gene expression in metabolites pyrimidine, and lipid metabolism in CIR rats and plays an active role in the treatment of CIR injury through multiple targets and pathways.
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Tipo de estudo: Estudo prognóstico Idioma: Inglês Revista: J Ethnopharmacol Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: China

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Tipo de estudo: Estudo prognóstico Idioma: Inglês Revista: J Ethnopharmacol Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: China