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Modification performance and electrochemical characteristics of different groups of modified aptamers applied for label-free electrochemical impedimetric sensors.
Nan, Mi-Na; Bi, Yang; Xue, Hua-Li; Long, Hai-Tao; Xue, Su-Lin; Pu, Lu-Mei; Prusky, Dov.
Afiliação
  • Nan MN; College of Science, Gansu Agricultural University, Lanzhou 730070, PR China; College of Horticulture, Gansu Agricultural University, Lanzhou 730070, PR China.
  • Bi Y; College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: biyang@gsau.edu.cn.
  • Xue HL; College of Science, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: xuehuali77@sina.com.
  • Long HT; College of Science, Gansu Agricultural University, Lanzhou 730070, PR China.
  • Xue SL; College of Horticulture, Gansu Agricultural University, Lanzhou 730070, PR China.
  • Pu LM; College of Science, Gansu Agricultural University, Lanzhou 730070, PR China.
  • Prusky D; College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China; Department of Postharvest Science of Fresh Produce, the Volcani Center, Agricultural Research Organization, Bet Dagan 50250, Israel.
Food Chem ; 337: 127761, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32777565
ABSTRACT
Amino and thiolated aptamers are the main aptamers used to construct label-free electrochemical impedimetric aptasensors. In this study, the modification performance and electrochemical properties of amino aptamers and thiolated aptamers were studied in the construction of label-free impedimetric sensors. The results showed that the initial modification density of amino aptamers was higher than that of thiol aptamers. Aptamers can recognize and bind OTA to generate electrical signals. The higher the density of aptamer modification was, the better the electric signals were. If only considering the initial modification density, amino aptamers were more suitable for the preparation of aptasensors than thiolated aptamers. However, the modification density of the amino aptamer decreased with the prolonged immersion time in 1 mM HCl solution, which suggests that the stability of this sensor was poor. However, the thiolated aptamer maintained relatively constant density and could be reused. Thus, the thiolated aptasensor had a wide range and good reproducibility and stability for the determination of ochratoxin A (OTA). In addition, this study proved that gold nanoparticles play an important role in signal amplification by increasing the effective gold surface to fix more aptamers in the process of sensor preparation.
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Food Chem Ano de publicação: 2021 Tipo de documento: Artigo

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Food Chem Ano de publicação: 2021 Tipo de documento: Artigo