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Tumor-cell detection, labeling and phenotyping with an electron-doped bifunctional signal-amplifier.
Lu, Hao; Jin, Dan; Zhu, Lifei; Guo, Tongtong; Li, Xinchun; Peng, Xin-Xin; Mo, Guoyan; Tang, Lina; Zhang, Guo-Jun; Yang, Fan.
Afiliação
  • Lu H; College of Pharmacy, School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, 430065, China.
  • Jin D; College of Pharmacy, School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, 430065, China.
  • Zhu L; College of Pharmacy, School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, 430065, China.
  • Guo T; School of Pharmacy, Guangxi Medical University, Nanning, 530021, China.
  • Li X; School of Pharmacy, Guangxi Medical University, Nanning, 530021, China. Electronic address: xchleegxmu@163.com.
  • Peng XX; College of Pharmacy, School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, 430065, China.
  • Mo G; College of Pharmacy, School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, 430065, China.
  • Tang L; College of Pharmacy, School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, 430065, China.
  • Zhang GJ; College of Pharmacy, School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, 430065, China.
  • Yang F; School of Pharmacy, Guangxi Medical University, Nanning, 530021, China. Electronic address: yangfan@gxmu.edu.cn.
Biosens Bioelectron ; 170: 112662, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-33032198
ABSTRACT
Cancer cell enumeration and phenotyping can predict the prognosis and the therapy efficacy in patients, yet it remains challenging to detect the rare tumor cells. Herein, we report an octopus-inspired, bifunctional aptamer signal amplifier-based cytosensor (OApt-cytosensor) for sensitive cell analysis. By assembling high-affinity antibodies on an electrode surface, the target cells could be specifically captured and thus been sandwiched by the cell surface marker-specific DNA aptamers. These on-cell aptamers function as electrochemical signal amplifiers by base-selective electronic doping with methylene blue. Such a sandwich configuration enables highly sensitive cell detection down to 10 cells/mL (equal to ~1-2 cells at a sampling volume of 150 µL), even in a large excess of nontarget blood cells. This approach also reveals the cell-surface markers and tracks the cellular epithelial-to-mesenchymal transition induced by signaling regulators. Furthermore, the electron-doped aptamer shows remarkable cell fluorescent labeling that guides the release of the captured cells from electrode surface via electrochemistry. These features make OApt-cytosensor a promising tool in revealing the heterogeneous cancer cells and anticancer drug screening at the single-cell level.
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Tipo de estudo: Estudo diagnóstico Idioma: Inglês Revista: Biosens Bioelectron Assunto da revista: Biotecnologia Ano de publicação: 2020 Tipo de documento: Artigo País de afiliação: China

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Tipo de estudo: Estudo diagnóstico Idioma: Inglês Revista: Biosens Bioelectron Assunto da revista: Biotecnologia Ano de publicação: 2020 Tipo de documento: Artigo País de afiliação: China