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Diagnostic and prognostic value of MCM3 and its interacting proteins in hepatocellular carcinoma.
Li, Hong-Tao; Wei, Bing; Li, Zhou-Quan; Wang, Xiao; Jia, Wen-Xian; Xu, Yan-Zhen; Liu, Jia-Yi; Shao, Meng-Nan; Chen, Sui-Xia; Mo, Nan-Fang; Zhao, Dong; Zuo, Wen-Pu; Qin, Jian; Li, Ping; Zhang, Qin-Le; Yang, Xiao-Li.
Afiliação
  • Li HT; Scientific Research Center, Guilin Medical University, Guilin, Guangxi 541100, P.R. China.
  • Wei B; College of International Education, Guilin Medical University, Guilin, Guangxi 541100, P.R. China.
  • Li ZQ; Scientific Research Center, Guilin Medical University, Guilin, Guangxi 541100, P.R. China.
  • Wang X; Scientific Research Center, Guilin Medical University, Guilin, Guangxi 541100, P.R. China.
  • Jia WX; Department of Pathology, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
  • Xu YZ; College of Pharmacy, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
  • Liu JY; Scientific Research Center, Guilin Medical University, Guilin, Guangxi 541100, P.R. China.
  • Shao MN; Department of Pathology, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
  • Chen SX; College and Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
  • Mo NF; Scientific Research Center, Guilin Medical University, Guilin, Guangxi 541100, P.R. China.
  • Zhao D; Scientific Research Center, Guilin Medical University, Guilin, Guangxi 541100, P.R. China.
  • Zuo WP; Scientific Research Center, Guilin Medical University, Guilin, Guangxi 541100, P.R. China.
  • Qin J; Scientific Research Center, Guilin Medical University, Guilin, Guangxi 541100, P.R. China.
  • Li P; Medical Scientific Research Center, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
  • Zhang QL; School of Public Health, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
  • Yang XL; Department of Pathology, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
Oncol Lett ; 20(6): 308, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33093917
ABSTRACT
Aberrant DNA replication is one of the driving forces behind oncogenesis. Furthermore, minichromosome maintenance complex component 3 (MCM3) serves an essential role in DNA replication. Therefore, in the present study, the diagnostic and prognostic value of MCM3 and its interacting proteins in hepatocellular carcinoma (HCC) were investigated. By utilizing The Cancer Genome Atlas (TCGA) database, global MCM3 mRNA levels were assessed in HCC and normal liver tissues. Its effects were further analyzed by reverse transcription-quantitative PCR (RT-qPCR), western blotting and immunohistochemistry in 78 paired HCC and adjacent tissues. Functional and pathway enrichment analyses were performed using the Search Tool for the Retrieval of Interacting Genes database. The expression levels of proteins that interact with MCM3 were also analyzed using the TCGA database and RT-qPCR. Finally, algorithms combining receiver operating characteristic (ROC) curves were constructed using binary logistic regression using the TCGA results. Increased MCM3 mRNA expression with high α-fetoprotein levels and advanced Edmondson-Steiner grade were found to be characteristic of HCC. Survival analysis revealed that high MCM3 expression was associated with poor outcomes in patients with HCC. In addition, MCM3 protein expression was associated with increased tumor invasion in HCC tissues. MCM3 and its interacting proteins were found to be primarily involved in DNA replication, cell cycle and a number of binding processes. Algorithms combining ROCs of MCM3 and its interacting proteins were found to have improved HCC diagnosis ability compared with MCM3 and other individual diagnostic markers. In conclusion, MCM3 appears to be a promising diagnostic biomarker for HCC. Additionally, the present study provides a basis for the multi-gene diagnosis of HCC using MCM3.
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Oncol Lett Ano de publicação: 2020 Tipo de documento: Artigo

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Revista: Oncol Lett Ano de publicação: 2020 Tipo de documento: Artigo
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