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Development and Expression of Subunit Vaccines Against Viruses in Plants.
Esqueda, Adrian; Chen, Qiang.
Afiliação
  • Esqueda A; The Biodesign Institute and School of Life Sciences, Arizona State University, Tempe, AZ, USA.
  • Chen Q; The Biodesign Institute and School of Life Sciences, Arizona State University, Tempe, AZ, USA. Qiang.Chen.4@asu.edu.
Methods Mol Biol ; 2225: 25-38, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33108655
ABSTRACT
Various systems exist for the robust production of recombinant proteins. However, only a few systems are optimal for human vaccine protein production. Plant-based transient protein expression systems offer an advantageous alternative to costly mammalian cell culture-based systems and can perform posttranslational modifications due to the presence of an endomembrane system that is largely similar to that of the animal cell. Technological advances in expression vectors for transient expression in the last two decades have produced new plant expression systems with the flexibility and speed that cannot be matched by those based on mammalian or insect cell culture. The rapid and high-level protein production capability of transient expression systems makes them the optimal system to quickly and versatilely develop and produce vaccines against viruses such as 2019-nCoV that have sudden and unpredictable outbreaks. Here, expression of antiviral subunit vaccines in Nicotiana benthamiana plants via transient expression is demonstrated.
Assuntos
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Plantas / Pneumonia Viral / Infecções por Coronavirus / Vacinas de Subunidades / Pandemias / Betacoronavirus Limite: Humanos Idioma: Inglês Revista: Methods Mol Biol Assunto da revista: Biologia Molecular Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: Estados Unidos

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Plantas / Pneumonia Viral / Infecções por Coronavirus / Vacinas de Subunidades / Pandemias / Betacoronavirus Limite: Humanos Idioma: Inglês Revista: Methods Mol Biol Assunto da revista: Biologia Molecular Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: Estados Unidos