What determines eukaryotic translation elongation: recent molecular and quantitative analyses of protein synthesis.
Open Biol
; 10(12): 200292, 2020 12.
Article
em En
| MEDLINE
| ID: mdl-33292102
ABSTRACT
Protein synthesis from mRNA is an energy-intensive and tightly controlled cellular process. Translation elongation is a well-coordinated, multifactorial step in translation that undergoes dynamic regulation owing to cellular state and environmental determinants. Recent studies involving genome-wide approaches have uncovered some crucial aspects of translation elongation including the mRNA itself and the nascent polypeptide chain. Additionally, these studies have fuelled quantitative and mathematical modelling of translation elongation. In this review, we provide a comprehensive overview of the key determinants of translation elongation. We discuss consequences of ribosome stalling or collision, and how the cells regulate translation in case of such events. Next, we review theoretical approaches and widely used mathematical models that have become an essential ingredient to interpret complex molecular datasets and study translation dynamics quantitatively. Finally, we review recent advances in live-cell reporter and related analysis techniques, to monitor the translation dynamics of single cells and single-mRNA molecules in real time.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Elongação Traducional da Cadeia Peptídica
/
Biossíntese de Proteínas
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Células Eucarióticas
Limite:
Animals
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Humans
Idioma:
En
Revista:
Open Biol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
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