Your browser doesn't support javascript.
loading
Temporal focusing multiphoton microscopy with optimized parallel multiline scanning for fast biotissue imaging.
Chang, Chia-Yuan; Lin, Chun-Yun; Hu, Yvonne Y; Tsai, Sheng-Feng; Hsu, Feng-Chun; Chen, Shean-Jen.
Afiliação
  • Chang CY; National Cheng Kung University, Department of Mechanical Engineering, Tainan, Taiwan.
  • Lin CY; National Chiao Tung University, College of Photonics, Tainan, Taiwan.
  • Hu YY; National Cheng Kung University, Department of Photonics, Tainan, Taiwan.
  • Tsai SF; National Cheng Kung University, Department of Cell Biology and Anatomy, Tainan, Taiwan.
  • Hsu FC; National Chiao Tung University, College of Photonics, Tainan, Taiwan.
  • Chen SJ; National Chiao Tung University, College of Photonics, Tainan, Taiwan.
J Biomed Opt ; 26(1)2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33386708
ABSTRACT

SIGNIFICANCE:

Line scanning-based temporal focusing multiphoton microscopy (TFMPM) has superior axial excitation confinement (AEC) compared to conventional widefield TFMPM, but the frame rate is limited due to the limitation of the single line-to-line scanning mechanism. The development of the multiline scanning-based TFMPM requires only eight multiline patterns for full-field uniform multiphoton excitation and it still maintains superior AEC.

AIM:

The optimized parallel multiline scanning TFMPM is developed, and the performance is verified with theoretical simulation. The system provides a sharp AEC equivalent to the line scanning-based TFMPM, but fewer scans are required.

APPROACH:

A digital micromirror device is integrated in the TFMPM system and generates the multiline pattern for excitation. Based on the result of single-line pattern with sharp AEC, we can further model the multiline pattern to find the best structure that has the highest duty cycle together with the best AEC performance.

RESULTS:

The AEC is experimentally improved to 1.7 µm from the 3.5 µm of conventional TFMPM. The adopted multiline pattern is akin to a pulse-width-modulation pattern with a spatial period of four times the diffraction-limited line width. In other words, ideally only four π / 2 spatial phase-shift scans are required to form a full two-dimensional image with superior AEC instead of image-size-dependent line-to-line scanning.

CONCLUSIONS:

We have demonstrated the developed parallel multiline scanning-based TFMPM has the multiline pattern for sharp AEC and the least scans required for full-field uniform excitation. In the experimental results, the temporal focusing-based multiphoton images of disordered biotissue of mouse skin with improved axial resolution due to the near-theoretical limit AEC are shown to clearly reduce background scattering.
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Assunto da revista: Engenharia Biomédica / Oftalmologia Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: Taiwan

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Idioma: Inglês Assunto da revista: Engenharia Biomédica / Oftalmologia Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: Taiwan