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Cas12a-based electrochemiluminescence biosensor for target amplification-free DNA detection.
Liu, Peng-Fei; Zhao, Kai-Ren; Liu, Zhi-Jun; Wang, Li; Ye, Shu-Ying; Liang, Guo-Xi.
Afiliação
  • Liu PF; School of Pharmacy, Jiangsu University, Zhenjiang, 212013, China.
  • Zhao KR; School of Pharmacy, Jiangsu University, Zhenjiang, 212013, China.
  • Liu ZJ; School of Pharmacy, Jiangsu University, Zhenjiang, 212013, China.
  • Wang L; School of Pharmacy, Jiangsu University, Zhenjiang, 212013, China. Electronic address: wangjill@126.com.
  • Ye SY; School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China.
  • Liang GX; School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China. Electronic address: gxliang@ujs.edu.cn.
Biosens Bioelectron ; 176: 112954, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33412428
ABSTRACT
CRISPR/Cas system have drawn increasing attention in accurate and sensitive nucleic acids detection. Herein, we reported a novel Cas12a-based electrochemiluminescence biosensor for target amplification-free human papilloma virus subtype (HPV-16) DNA detection. During this detection process, Cas12a employed its two-part recognition mechanism to improve the specificity and trans-cleavage capability to achieve signal amplification, while L-Methionine stabilized gold nanoclusters (Met-AuNCs) were served as high-efficiency ECL emitters to achieve ECL signal transition. Given the unique combination of Cas12a with ECL technique, the detection limit was determined as 0.48 pM and the whole detection could be completed within 70 min. We also validated the practical application of the proposed biosensor by using undiluted human blood samples, which gives impetus to the design of new generations of CRISPR/Cas detection system beyond the traditional ones with ultimate applications in sensing analysis and diagnostic technologies.
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Tipo de estudo: Estudo diagnóstico Idioma: Inglês Revista: Biosens Bioelectron Assunto da revista: Biotecnologia Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: China

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Tipo de estudo: Estudo diagnóstico Idioma: Inglês Revista: Biosens Bioelectron Assunto da revista: Biotecnologia Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: China