Green synthesized silver nanoparticles obtained from Stachys schtschegleevii extract: ct-DNA interaction and in silico and in vitro investigation of antimicrobial activity.
J Biomol Struct Dyn
; 41(6): 2175-2188, 2023 04.
Article
em En
| MEDLINE
| ID: mdl-35048781
ABSTRACT
The aim of this study was the synthesis of Ag nanoparticles (AgNPs) by using Stachys schtschegleevii extract and checking the composition, morphology and size of the green synthesized AgNPs using the analytical techniques (UV-vis, DLS, zeta potential, SEM-EDX, FT-IR and TEM). The TEM images of AgNPs represent a smooth surface and are spherical in shape with an average particle size of 31.43 nm. The antioxidant activities of green synthesized AgNPs were appraised by radical scavenging 1, 1-diphenyl-2-picrylhydrazyl test and the green synthesized AgNPs showed a strong ability to scavenge free radicals. In addition, AgNPs displayed a remarkable antibacterial and antifungal activity against various microorganisms. We employed molecular docking to investigate the AgNPs interaction with Dihydrofolate reductase (DHFR) of Escherichia coli, Staphylococcus aureus, and Candida albicans and there is a good agreement between molecular docking and our experimental results. The result of ct-DNA-AgNPs interaction demonstrated that AgNPs can bind to ct-DNA through partial intercalation binding mode.Communicated by Ramaswamy H. Sarma.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Stachys
/
Nanopartículas Metálicas
Idioma:
En
Revista:
J Biomol Struct Dyn
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Irã
País de publicação:
ENGLAND
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ESCOCIA
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GB
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GREAT BRITAIN
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INGLATERRA
/
REINO UNIDO
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SCOTLAND
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UK
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UNITED KINGDOM