Association of HLA-DRB1 alleles with rheumatoid arthritis in Split-Dalmatia County in southern Croatia.
Wien Klin Wochenschr
; 134(11-12): 463-470, 2022 Jun.
Article
em En
| MEDLINE
| ID: mdl-35238988
ABSTRACT
OBJECTIVE:
The aim of this study was to investigate the distribution of HLA-DRB1 alleles in patients with rheumatoid arthritis (RA) in the Sinj Region (SR) and the rest of the Split-Dalmatia County (SDC) in Croatia and to determine their relationship with disease severity.METHODS:
A total of 74 RA patients and 80 healthy controls from the SR, and 74 RA patients and 80 healthy controls from the rest of the SDC were genotyped using sequence-specific oligonucleotide primed PCR. High-resolution typing of HLA-DRB1*04 alleles was performed using the single specific primed polymerase chain reaction (PCR-SSP) method. Serum anti-CCP, rheumatoid factor, Creactive protein, and erythrocyte sedimentation rate were measured in all RA patients, whereas disease activity was assessed by DAS-28 and functional status by the Health Assessment Questionnaire Disability Index.RESULTS:
The HLA-DRB1*04 allele was more frequent in patients with RA from the SR than that in patients from the rest of the SDC (18.2% vs. 9.5%; Pâ¯= 0.014), whereas the HLA-DRB1*15 allele was more frequent in patients with RA from the rest of the SDC than in patients from the SR (16.2% vs. 7.4%; Pâ¯= 0.010). Shared epitope (SE) positive patients from the SR had significantly higher serum anti-CCP and RF antibody levels (Pâ¯= 0.014 and Pâ¯= 0.004, respectively), higher disease activity (Pâ¯= 0.043), and worse functional status (Pâ¯< 0.001), than SE-positive patients from the rest of the SDC.CONCLUSION:
The observed higher incidence of more severe forms of RA in the SR in comparison to the rest of the SDC might be associated with the higher incidence of HLA-DRB1*04 allele in the SR.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Artrite Reumatoide
/
Cadeias HLA-DRB1
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
País/Região como assunto:
Europa
Idioma:
En
Revista:
Wien Klin Wochenschr
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Croácia
País de publicação:
AT
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AUSTRIA
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ÁUSTRIA