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Involvement of oxidative stress-related apoptosis in chlorpyrifos-induced cytotoxicity and the ameliorating potential of the antioxidant vitamin E in human glioblastoma cells.
Hsu, Shu-Shong; Lin, Yung-Shang; Chen, Hui-Ching; Liang, Wei-Zhe.
Afiliação
  • Hsu SS; Department of Neurosurgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
  • Lin YS; Department of Neurosurgery, National Defense Medical Center, Taipei, Taiwan.
  • Chen HC; College of Health and Nursing, Meiho University, Pingtung, Taiwan.
  • Liang WZ; Department of Neurosurgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Environ Toxicol ; 38(9): 2143-2154, 2023 Sep.
Article em En | MEDLINE | ID: mdl-37283489
ABSTRACT
Organophosphate pesticides (OPs), which are among the most widely used synthetic chemicals for the control of a wide variety of pests, are however associated with various adverse reactions in animals and humans. Chlorpyrifos, an OP, has been shown to cause various health complications due to ingestion, inhalation, or skin absorption. The mechanisms underlying the adverse effect of chlorpyrifos on neurotoxicity have not been elucidated. Therefore, we aimed to determine the mechanism of chlorpyrifos-induced cytotoxicity and to examine whether the antioxidant vitamin E (VE) ameliorated these cytotoxic effects using DBTRG-05MG, a human glioblastoma cell line. The DBTRG-05MG cells were treated with chlorpyrifos, VE, or chlorpyrifos plus VE and compared with the untreated control cells. Chlorpyrifos induced a significant decrease in cell viability and caused morphological changes in treated cultures. Furthermore, chlorpyrifos led to the increased production of reactive oxygen species (ROS) accompanied by a decrease in the level of reduced glutathione. Additionally, chlorpyrifos induced apoptosis by upregulating the protein levels of Bax and cleaved caspase-9/caspase-3 and by downregulating the protein levels of Bcl-2. Moreover, chlorpyrifos modulated the antioxidant response by increasing the protein levels of Nrf2, HO-1, and NQO1. However, VE reversed the cytotoxicity and oxidative stress induced by chlorpyrifos treatment in DBTRG-05MG cells. Overall, these findings suggest that chlorpyrifos causes cytotoxicity through oxidative stress, a process that may play an important role in the development of chlorpyrifos-associated glioblastoma.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vitamina E / Clorpirifos / Inseticidas / Antioxidantes Limite: Animals / Humans Idioma: En Revista: Environ Toxicol Assunto da revista: SAUDE AMBIENTAL / TOXICOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Taiwan País de publicação: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vitamina E / Clorpirifos / Inseticidas / Antioxidantes Limite: Animals / Humans Idioma: En Revista: Environ Toxicol Assunto da revista: SAUDE AMBIENTAL / TOXICOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Taiwan País de publicação: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA