A review of tamoxifen administration regimen optimization for Cre/loxp system in mouse bone study.
Biomed Pharmacother
; 165: 115045, 2023 Sep.
Article
em En
| MEDLINE
| ID: mdl-37379643
ABSTRACT
Gene knockout is a technique routinely used in basic experimental research, particularly in mouse skeletal and developmental studies. Tamoxifen-induced Cre/loxp system is known for its temporal and spatial precision and commonly utilized by researchers. However, tamoxifen has been shown its side effects on affecting the phenotype of mouse bone directly. This review aimed to optimize tamoxifen administration regimens including its dosage and duration, to identify an optimal induction strategy that minimizes potential side effects while maintaining recombination efficacy. This study will help researchers in designing gene knockout experiments in bone when using tamoxifen.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tamoxifeno
/
Integrases
Limite:
Animals
Idioma:
En
Revista:
Biomed Pharmacother
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
China