Specific binding of G-quadruplex in SARS-CoV-2 RNA by RHAU peptide.
Curr Res Struct Biol
; 7: 100126, 2024.
Article
em En
| MEDLINE
| ID: mdl-38292819
ABSTRACT
G-quadruplexes (G4s) are reported to present on the SARS-CoV-2 RNA genome and control various viral activities. Specific ligands targeting those viral nucleic acid structures could be investigated as promising detection methods or antiviral reagents to suppress this menacing virus. Herein, we demonstrate the binding between a G4 structure in the RNA of SARS-CoV-2 and a fluorescent probe created by fusing a parallel-G4 specific RHAU53 and a cyan fluorescent protein. The specific binding of G4 in SARS-CoV-2 by RHAU peptide was easily detected under the fluorescence spectrometer. The drawbacks of this approach and potential solutions are also discussed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Curr Res Struct Biol
Ano de publicação:
2024
Tipo de documento:
Article
País de publicação:
Holanda