Point-of-Care Fluorescence Biosensing System for Rapid Multi-Allergen Screening.
Sensors (Basel)
; 24(11)2024 May 21.
Article
em En
| MEDLINE
| ID: mdl-38894075
ABSTRACT
With the steady increase in allergy prevalence worldwide, there is a strong need for novel diagnostic tools for precise, fast, and less invasive testing methods. Herein, a miniatured fluorescence-based biosensing system is developed for the rapid and quantitative detection of allergen-specific immunoglobulin-E. An antibody-based fluorescence assay in a microfluidic-patterned slide, combined with a custom-made portable fluorescence reader for image acquisition and user-friendly software for the data analysis, enables obtaining results for multiple allergens in just ~1 h with only 80 µL of blood serum. The multiplexed detection of common birch, timothy grass, cat epithelia, house dust mite, and dog epithelia shows quantitative IgE-mediated allergic responses to specific allergens in control serum samples with known total IgE concentration. The responses are verified with different control tests and measurements with a commercial fluorescence reader. These results open the door to point-of-care allergy screening for early diagnosis and broader access and for large-scale research in allergies.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina E
/
Alérgenos
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Técnicas Biossensoriais
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Sistemas Automatizados de Assistência Junto ao Leito
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Sensors (Basel)
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Suíça
País de publicação:
CH
/
SUIZA
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SUÍÇA
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SWITZERLAND