Continuous Flow Synthesis of Hexyl Laurate Using Immobilized Thermomyces Lanuginosus Lipase from Residual Babassu Mesocarp.
Chempluschem
; : e202400442, 2024 Aug 06.
Article
em En
| MEDLINE
| ID: mdl-39105675
ABSTRACT
This work applied BM as support for immobilization of lipase TLL in packed-bed reactor and its application for the synthesis of hexyl laurate. Initially, the percolation of a solution containing 5 mg of TLL at 25 oC generated an immobilized derivative with hydrolytic activity of 504.7 U/g and 31.7% of recovered activity. Subsequent treatment with n-hexane, as well as the effect of temperature on the immobilization process were able to improve the activities of the final BM-TLLF, achieving a hydrolysis activity of 7023 U/g and esterification activity of 430 U/g against 142 U/g and 113.5 U/g respectively presented by commercial TLIM. Desorption studies showed that the TL IM has 18 mg of protein per gram of support, compared to 4.92 mg presented by BM-TLL. Both biocatalysts were applied to synthesize hexyl laurate, achieving 98% conversion at 40°C within a residence time of 2 hours. Notably, BM-TLLF displayed exceptional recyclability, maintaining catalytic efficiency over 12 cycles. This reflects a productivity of 180 mg of product/h/U of the enzyme, surpassing 46 mg/h/U obtained for TLIM. These results demonstrate the efficacy of continuous flow technology in creating a competitive and integrated process offering an exciting alternative for the valorization of residual lignocellulosic biomass.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Chempluschem
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Alemanha