Photometric measurement of lipoprotein-cholesterol after agarose electrophoresis: comparison with single-spin ultracentrifugal analysis.
Clin Chim Acta
; 111(2-3): 239-45, 1981 Apr 09.
Article
em En
| MEDLINE
| ID: mdl-7226552
ABSTRACT
A method is presented for the evaluation of lipoprotein-cholesterol after fractionation of 20 microliter of serum in agarose electrophoresis in a discontinuous buffer system. The prestained lipoprotein bands are separated in less tha 1 h, cut out and extracted with butanol. The cholesterol in the extract is measured by a simple colorimetric method. The recoveries of lipoprotein cholesterol were 95.3% +/- 13.0 (S.D.), and the average coefficient of variation was 8% for alphalipoprotein, 6.4% for betalipoprotein and 8.0% for pre-betalipoproteins. Lipoprotein cholesterol concentrations, determined by the proposed method correlated well with those analyzed following ultracentrifugation. The method can handle up to 4 serum samples run in duplicate per day and only requires equipment available in conventional clinical laboratories, and stable reagents.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Colesterol
/
Lipoproteínas
Limite:
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Clin Chim Acta
Ano de publicação:
1981
Tipo de documento:
Article
País de publicação:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS