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Cloning and expression of the Bacillus sphaericus 2362 mosquitocidal genes in a non-toxic unicellular cyanobacterium, Synechococcus PCC6301.
Appl Microbiol Biotechnol ; 47(4): 379-84, 1997 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-9163952
ABSTRACT
Genes encoding the mosquitocidal binary toxin of Bacillus sphaericus 2362 were introduced into Synechococcus PCC6301, a cyanobacterium that can tolerate a number of potential variations in the mosquito breeding environment, and can serve as a food source for mosquito larvae. The toxin genes, preceded by a Synechococcus rbcL promoter, were located on a mobilizable Escherichia coli Synechococcus shuttle vector, which was introduced into Synechococcus PCC6301 at frequencies of 10(-5)-10(-7) exconjugants/recipient, depending on the selective conditions used. Recombinant Synechococcus exhibited significant toxicity against 2-day-old and 6-day-old Culex quinquefasciatus larvae, the concentration required to kill 50% of larvae (LC50) being 2.1 x 10(5) and 1.3 x 10(5) cells/ml respectively. Mosquitocidal activity decreased tenfold after 20 generations of non-selective growth.
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Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Bacillus / Toxinas Bacterianas / Regulação Bacteriana da Expressão Gênica / Clonagem Molecular Limite: Animais Idioma: Inglês Revista: Appl Microbiol Biotechnol Ano de publicação: 1997 Tipo de documento: Artigo País de afiliação: Austrália