Application of an immunoperoxidase monolayer assay for the detection of arboviral antibodies.
J Virol Methods
; 65(2): 147-51, 1997 May.
Article
em En
| MEDLINE
| ID: mdl-9186937
ABSTRACT
An immunoperoxidase monolayer assay (IPMA) was adapted for the detection of antibodies to six arboviruses three viruses within the flavivirus group (dengue 2, West Nile (WN) and yellow fever) and three in the phlebovirus group (Rift Valley fever (RVF), sandfly fever Naples and sandfly fever Sicilian). Antibody titers of homologous hyper-immune mouse ascitic fluid (HMAF) measured by IPMA were two to eight-fold less than those determined by ELISA. In tests with heterologous HMAF, cross-reactions frequently observed in ELISA, particularly in the flavivirus group, were absent in all IPMA titrations. With human serum samples tested for antibodies to RVF (n = 52) and WN (n = 90), the sensitivity of IPMA as compared with ELISA was 96 and 91%, respectively, specificity of IPMA was 100%. In addition, the IPMA format has several advantages that make it a useful alternative to ELISA for diagnosing arboviral infections under field conditions.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arbovírus
/
Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
/
Prevalence_studies
/
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Egito
País de publicação:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS