A spectrum of mutations induced by crotonaldehyde in shuttle vector plasmids propagated in human cells.
Carcinogenesis
; 19(1): 69-72, 1998 Jan.
Article
em En
| MEDLINE
| ID: mdl-9472695
ABSTRACT
A spectrum of crotonaldehyde-induced mutations in the supF gene of the shuttle vector plasmid pMY189 replicated in human fibroblast cells was examined. Base sequence analysis of 104 plasmids with mutations in the supF gene revealed that the majority of the mutations were base substitutions (85%) and the rest were frameshifts (15%). A single base substitution was most frequently found (47%), while 25% had multiple base substitutions and interestingly 13% had tandem (adjacent two) base substitutions. Of the base substitution mutations, 50% were GC-->TA transversions and 23% were GC-->AT transitions. The mutations were not distributed randomly but were located at several hotspots, most of which were GC base pairs in 5'-AAGG-3' (or 5'-CCTT-3') sequences. Production of propanodeoxyguanosine adducts may be related to such specificity in the mutation spectrum.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA de Transferência
/
Mutação da Fase de Leitura
/
Mutação Puntual
/
Aldeídos
/
Mutagênicos
Limite:
Humans
Idioma:
En
Revista:
Carcinogenesis
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Japão