Polymerase chain reaction in the diagnosis of tuberculosis. Comparison of two target sequences for amplification.
Zentralbl Bakteriol
; 282(4): 353-61, 1995 Oct.
Article
em En
| MEDLINE
| ID: mdl-9810656
ABSTRACT
Amplification of a 340 bp sequence of the 38 kDa protein gene of Mycobacterium tuberculosis by the polymerase chain reaction has been developed. The sensitivity of this PCR was shown to be 10 fg both by agarose gel electrophoresis and Southern blot hybridisation being equivalent to 2-3 organisms and highly specific to M. tuberculosis and excluding even M. tuberculosis H37Ra and Mycobacterium bovis BCG. Sputum samples from patients with pulmonary tuberculosis gave a positivity rate of 45%. PCR was also performed using pt8 and pt9 primers which amplified a 541 bp sequence of IS6110. 41% of the above samples gave positive amplification. Three samples that were positive for 38 kDa sequence were negative for IS6110.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tuberculose
/
Reação em Cadeia da Polimerase
/
Mycobacterium tuberculosis
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Zentralbl Bakteriol
Assunto da revista:
BACTERIOLOGIA
/
MICROBIOLOGIA
/
PARASITOLOGIA
/
VIROLOGIA
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Índia
País de publicação:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY