High human T-cell lymphotropic virus type I proviral DNA load with polyclonal integration in peripheral blood mononuclear cells of French West Indian, Guianese, and African patients with tropical spastic paraparesis
Blood
; 75(2): 428-33, Jan. 15, 1990.
Artigo
em Inglês
| MedCarib
| ID: med-10028
Biblioteca responsável:
JM3.1
Localização: JM3.1; RB145.A1B5
ABSTRACT
Human T-cell lymphotropic virus type I (HTLV-I) proviral integration status was examined by Southern blot analysis in peripheral blood mononuclear cell (PBMC) DNA from patients presenting a tropical spastic paraparesis (TSP) and serological evidence of HTLV-I infection. Surface phenotype and morphological aspects of PBMC were also studied. A polyclonal HTLV-I proviral integration was found in the PBMC of the 10 patients studied irrespective of their geographical origin (French West Indies, French Guiana, and Africa), the duration of their clincal illness, or the HTLV-I antibody titer. Furthermore, by dilution experiments and hypothesizing that only one copy of HTLV-I proviral DNA is present in one call, we estimated that this HTLV-I integration is present in 3 percent to 15 percent of their PBMC. All 10 TSP/HTLV-I patients studied had an average of 10 percent of thier lymphocytes abnormal, presening either a misshapen nucleus or an adult T-cell leukemia/lymphoma(ATL)-like feature. Moreover, an elevated CD4/CD8 ratio associated with the presence of activated T cells with a high level of DR expression was observed in most patients. The significant frequency of viral-positive PBMC and the important load of HTLV-I proviral DNA that we observed in TSP/HTLV-I patients might play an important role in the pathogenesis of this recently identified clinico-virological entity. (AU)
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Coleções:
Bases de dados internacionais
Base de dados:
MedCarib
Assunto principal:
Leucócitos Mononucleares
/
Vírus Linfotrópico T Tipo 1 Humano
/
Paraparesia Espástica Tropical
Limite:
Humanos
País/Região como assunto:
África
/
América do Sul
/
Caribe
/
Guiana Francesa
/
Martinica
Idioma:
Inglês
Revista:
Blood
Ano de publicação:
1990
Tipo de documento:
Artigo