Sodium azide preservation of faecal specimens for Kato analysis
Parasitology
; 90: 463-9, 1985.
Artigo
em Inglês
| MedCarib
| ID: med-12182
Biblioteca responsável:
JM3.1
Localização: JM9.1; QL757.A1P37
ABSTRACT
The modified Kato technique has the advantages of reproducibility, simplicity and economy the disadvantage is that it cannot be used in conjunction with traditional faecal preservatives. Sodium azide has been evaluated as a preservative for human faeces for subsequent Kato analysis. More than 400 faecal samples (from normal and malnourished children, and from mixed-age participants in a field survey of the Turks and Caicos Islands) were each mixed with 2-5mg of sodium azide powder and stored in 2 or 4ml autoanalyser cups at ambient tropical temperature. At intervals up to 30 weeks, aliquots were prepared for Kato analysis. Trichuria trichiura, Ascaris lumbricoides and Necator americanus eggs were well preserved without degenerative or developmental changes in morphology. Quantitative analyses of 18 samples indicated that the mean egg count/sample did not change significantly after storage for 1, 2, 4, 8, 12 and 16 weeks in preservative. The use of aside preservative extends the applications of the Kato technique to field and clinical studies in which delays may occur between specimen collection and examination. The direct costs of azide preservation are substantially lower than for traditional methods and the preserved specimens are more compact and resistant to leakage. (Summary)
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Coleções:
Bases de dados internacionais
Base de dados:
MedCarib
Assunto principal:
Preservação Biológica
/
Azidas
/
Fezes
/
Nematoides
Limite:
Criança
/
Humanos
Idioma:
Inglês
Revista:
Parasitology
Ano de publicação:
1985
Tipo de documento:
Artigo