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Total protein synthesis in elderly people: a comparison of results with [15N]glycine and [14C]leucine
Clin Sci Mol Med ; 53(3): 277-88, Sep 1977.
Artigo em Inglês | MedCarib | ID: med-12400
Biblioteca responsável: JM3.1
Localização: JM3.1; R31.C56
ABSTRACT
Total body protein turnover was studied in six elderly patients. During the study they were fed by continuous infusion of a liquid formula through a nasogastric tube. L-[1-1+C] leucine and [15N]-glycine were infused at a constant rate for 30 h. The labelled glycine was infused into the intragastric line; the labelled leucine was given either by this route of intravenously. The specific radioactivity of free leucine in plasma and the rate of output of 14CO2 in expired air both reached a plateau at 10 h, and remained constant until the end of the infusion at 30 h. The 15N abundance in urinary urea and total N was very similar. In neither was a plateau reached by 30 h but in four out of the six patients the abundance in urinary NH4+ had attained a plateau by the end of the infusion. Flux rates and rates of protein synthesis were calculated in four ways (A) from the specific radioactivity of plasma leucine at plateau; (B) from the proportion of dose excreted as 1+CO2 at plateau; (C) from the final rates of 15N excretion in urea or total urinary N; (D) from the final or plateau rates of 15N excretion in urinary NH4+. On average, the estimates of synthesis rate obtained by methods B and C agreed closely; those given by methods A and D were lower.(AU)
Assuntos
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Coleções: Bases de dados internacionais Base de dados: MedCarib Assunto principal: Proteínas / Glicina / Leucina Limite: Idoso / Feminino / Humanos / Masculino Idioma: Inglês Revista: Clin Sci Mol Med Ano de publicação: 1977 Tipo de documento: Artigo
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Coleções: Bases de dados internacionais Base de dados: MedCarib Assunto principal: Proteínas / Glicina / Leucina Limite: Idoso / Feminino / Humanos / Masculino Idioma: Inglês Revista: Clin Sci Mol Med Ano de publicação: 1977 Tipo de documento: Artigo
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