Serologic responses, biosafety and clearance of four dosages of Brucella abortus strain RB51 in 6-10 months old water buffalo (Bubalus bubalis)
Veterinary immunology and immunopathology
; 109(1-2): 43-55, Jan. 2006. tab
Artigo
em Inglês
| MedCarib
| ID: med-17393
Biblioteca responsável:
TT5
Localização: TT5; W1, VE931HJ
ABSTRACT
Thirty water buffalo were obtained from a brucellosis-free farm in order to evaluate antibody responses, bacterial clearance and safety to Brucella abortus strain RB51 vaccine in a dose response study. The animals were randomly divided into five treatment groups. Groups IV received the recommended dose of RB51 vaccine (RD) once, RD twice 4 weeks apart, double RD once, double RD twice 4 weeks apart and saline once, respectively. Antibody responses to RB51 were monitored at 2, 4, 6, 8, 10, 12, 16 18, 22, 24 and 27 post-initial-inoculation weeks (PIW). Clearance of RB51 from the prescapular lymph node was evaluated at 2, 4, 6, 12, 18 and 24 PIW for groups 1, III and V and at 6, 8, 10, 16, 22 and 27 PIW for groups II and IV. To evaluate shedding of the RB51 strain, nasal, conjunctival, vaginal or preputial swabs were taken from all experimental animals at 1, 2, 3, 4, 6, 8 and 12 PIW. Sera taken at all PIW were negative for field strain B. abortus by both the buffered plate agglutination test (BPAT) and competitive enzyme-linked immunosorbent assay (c-ELISA). Antibody responses to RB51 were demonstrated in all vaccinates but not in the controls, up to 12 PIW, by complement fixation test (CFT) and the dot-blot assay with an 83.7% agreement for both tests. Clearance of RB51 occurred between 6 and 12 PIW in group I but less than 2 weeks after booster vaccinations in groups II and IV and between 4 and 6 PIW in group III. RB51 was not recovered at any time from swabs obtained from either RB51-vaccinates or non-vaccinates. The results of this study indicate that serologic responses to RB51 vaccination can be monitored by both CFT and dot-blot assay in water buffalo. Our data also indicates that RB51 vaccination does not interfere with brucellosis sero-surveillance and is safe (no serological and bacteriological evidence of spread to non-vaccinates, no adverse clinical signs or detectable abnormalities on haematology and serum biochemistry) for use in water buffalo (AU)
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Coleções:
Bases de dados internacionais
Contexto em Saúde:
ODS3- Meta 3D Reforçar a capacidade de alerta precoce, redução e gestão de riscos de saúde nacionais e globais
/
ODS3 - Saúde e Bem-Estar
/
Doenças Negligenciadas
/
ODS3 - Meta 3.3 Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
Problema de saúde:
Riscos Biológicos
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Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
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Doenças Negligenciadas
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Zoonoses
/
Brucelose
Base de dados:
MedCarib
Assunto principal:
Brucella abortus
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Búfalos
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Vacinação
Limite:
Animais
Idioma:
Inglês
Revista:
Veterinary immunology and immunopathology
Ano de publicação:
2006
Tipo de documento:
Artigo
Instituição/País de afiliação:
Istituto Superiore di Sanita/Italy
/
The University of the West Indies/Trinidad and Tobago