Phylogeography and molecular evolution of dengue 2 in the Caribbean basin, 19812000
Virology
; 324(1): 48-59, June 2004. mapastab^cilus
Artigo
em Inglês
| MedCarib
| ID: med-17551
Biblioteca responsável:
TT5
ABSTRACT
We sequenced the envelope (E) genes of 59 DEN-2 isolates collected from ten Caribbean islands, six South American countries, and two Central American countries between 1981 and 2000, a period characterized by hyperendemicity and increased incidence of severe dengue. Fifty-two isolates belonged to American/Asian subtype IIIb, possessing a characteristic polar residue at envelope aa position 390 (N [n = 48] or S [n = 4]) common to that group. Six isolates from Trinidad (1981), Honduras (1991 [4]), and El Salvador (1987) fell into the Native American subtype V (D at aa 390), and one from Honduras (1986) belonged to Asian subtype I. The data suggest that after its first isolation in the Caribbean in 1981, genotype IIIb spread throughout the Americas and effectively replaced subtype V throughout the Caribbean basin. The strain also evolved into several distinct lineages, based on substitutions in the E glycoprotein (amino acids 91 and 131), two of which were still in circulation in 2000. Interestingly, a molecular clock did not fit the data well, suggesting that other sources of rate variation, such as differential selection or differences in effective population sizes, may exist among lineages. Our results indicate the importance of large temporal- and geographical-scale phylogenetic studies in understanding disease dynamics, particularly where replacements between regions can occur.
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Coleções:
Bases de dados internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Dengue
Base de dados:
MedCarib
Assunto principal:
Região do Caribe
/
Evolução Molecular
/
Dengue
/
Flavivirus
Limite:
Animais
Idioma:
Inglês
Revista:
Virology
Ano de publicação:
2004
Tipo de documento:
Artigo
Instituição/País de afiliação:
Caribbean Epidemiology Centre/Trinidad and Tobago
/
University of Puerto Rico/Puerto Rico
/
University of the West Indies/Trinidad and Tobago