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Size heterogeneity in the 3' noncoding region of South American isolates of yellow fever virus
Bryant, Juliet E. ; Vasconcelos, Pedro F. C. ; Rijnbrand, Rene C. A. ; Mutebi, J. P. ; Higgs, Stephen; Barrett, Alan D. T. .
Afiliação
  • Bryant, Juliet E. ; University of Texas. Medical Branch. Department of Pathology. Texas. United States of America
  • Vasconcelos, Pedro F. C. ; Ministry of Health. Instituto Evandro Chagas. Department of Arbovirology. Belem. Brazil
  • Rijnbrand, Rene C. A. ; University of Texas. Medical Branch. Department of Pathology. Texas. United States of America
  • Mutebi, J. P. ; Chicago Department of Public Health. Chicago. United States of America
  • Higgs, Stephen; University of Texas. Medical Branch. Department of Pathology. Texas. United States of America
  • Barrett, Alan D. T. ; University of Texas. Medical Branch. Department of Pathology. Texas. United States of America
Journal of virology ; 79(6): 3807-3821, Mar. 2005. graf, tab, ilus
Artigo em Inglês | MedCarib | ID: med-17658
Biblioteca responsável: TT5
Localização: TT5; W1, JO97V
ABSTRACT
The 3' noncoding region (3' NCR) of flaviviruses contains secondary and tertiary structures essential for virus replication. Previous studies of yellow fever virus (YFV) and dengue virus have found that modifications to the 3' NCR are sometimes associated with attenuation in vertebrate and/or mosquito hosts. The 3' NCRs of 117 isolates of South American YFV have been examined, and major deletions and/or duplications of conserved RNA structures have been identified in several wild-type isolates. Nineteen isolates (designated YF-XL isolates) from Brazil, Trinidad, and Venezuela, dating from 1973 to 2001, exhibited a 216-nucleotide (nt) duplication, yielding a tandem repeat of conserved hairpin, stem-loop, dumbbell, and pseudoknot structures. YF-XL isolates were found exclusively within one subclade of South American genotype I YFV. One Brazilian isolate exhibited, in addition to the 216-nt duplication, a deletion of a 40-nt repeated hairpin (RYF) motif (YF-XL-DeltaRYF). To investigate the biological significance of these 3' NCR rearrangements, YF-XL-DeltaRYF and YF-XL isolates, as well as other South American YFV isolates, were evaluated for three phenotypes growth kinetics in cell culture, neuroinvasiveness in suckling mice, and ability to replicate and produce disseminated infections in Aedes aegypti mosquitoes. YF-XL-DeltaRYF and YF-XL isolates showed growth kinetics and neuroinvasive characteristics comparable to those of typical South American YFV isolates, and mosquito infectivity trials demonstrated that both types of 3' NCR variants were capable of replication and dissemination in a laboratory-adapted colony of A. aegypti.
Assuntos
Texto completo: Disponível Coleções: Bases de dados internacionais Contexto em Saúde: Doenças Negligenciadas Problema de saúde: Dengue Base de dados: MedCarib Assunto principal: Filogenia / Variação Genética / Dados de Sequência Molecular / Sequência de Bases / Células Cultivadas / Aedes / Conformação de Ácido Nucleico Tipo de estudo: Estudo prognóstico Limite: Animais / Humanos País/Região como assunto: América do Sul / Brasil / Caribe Inglês / Trinidad e Tobago / Venezuela Idioma: Inglês Revista: Journal of virology Ano de publicação: 2005 Tipo de documento: Artigo Instituição/País de afiliação: Chicago Department of Public Health/United States of America / Ministry of Health/Brazil / University of Texas/United States of America
Texto completo: Disponível Coleções: Bases de dados internacionais Contexto em Saúde: Doenças Negligenciadas Problema de saúde: Dengue Base de dados: MedCarib Assunto principal: Filogenia / Variação Genética / Dados de Sequência Molecular / Sequência de Bases / Células Cultivadas / Aedes / Conformação de Ácido Nucleico Tipo de estudo: Estudo prognóstico Limite: Animais / Humanos País/Região como assunto: América do Sul / Brasil / Caribe Inglês / Trinidad e Tobago / Venezuela Idioma: Inglês Revista: Journal of virology Ano de publicação: 2005 Tipo de documento: Artigo Instituição/País de afiliação: Chicago Department of Public Health/United States of America / Ministry of Health/Brazil / University of Texas/United States of America
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