The prevalence of Chlamydia trachomatis in fresh tissue specimens from patients with ectopic pregnancy or tubal factor infertility as determined by PCR and in-situ hybridisation
Journal of medical microbiology
; 50(10): 902-908, Oct. 2001. tab
Artigo
em Inglês
| MedCarib
| ID: med-17773
Biblioteca responsável:
TT5
ABSTRACT
The prevalence of chlamydial DNA determined by PCR and in-situ hybridisation (ISH) in fresh tissue specimens (endometrium, fallopian tube and ovary) was investigated in 33 women presenting with ectopic pregnancy (EP), 14 women with tubal factor infertility (TFI) and 50 control patients from the UK and the West Indies. In the UK EP group, chlamydial DNA was detected by PCR in 56% of patients; similar results were found in the Trinidad EP group (67%). In the TFI group, chlamydial DNA was detected in (71%) of patients by PCR. The detection of Chlamydia trachomatis DNA by ISH was highest in the TFI group (43%). Women presenting with EP and TFI showed evidence of previous or current genital C. trachomatis infection, underlining the importance of this microorganism in the development of these conditions. Importantly, chlamydial DNA could be detected in DNA preparations from the endometrium, fallopian tube and ovary of EP and TFI patients at the time of surgery.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
MedCarib
Assunto principal:
Infecções por Chlamydia
/
Chlamydia trachomatis
/
Genitália Feminina
Tipo de estudo:
Estudo de prevalência
/
Fatores de risco
Limite:
Adulto
/
Feminino
/
Humanos
País/Região como assunto:
Caribe Inglês
/
Europa
/
Trinidad e Tobago
Idioma:
Inglês
Revista:
Journal of medical microbiology
Ano de publicação:
2001
Tipo de documento:
Artigo
Instituição/País de afiliação:
Royal Liverpool and Broadgreen University Hospitals/United Kingdom
/
The University of the West Indies/Trinidad and Tobago
/
University of Bristol/United Kingdom
/
University of Sheffield Medical School/United Kingdom