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Fourth-generation enzyme-linked immunosorbent assay for the simultaneous detection of human immunodeficiency virus antigen and antibody
Saville, Rebecca D. ; Constantine, Niel T. ; Cleghorn, Farley R. ; Jack, Noreen; Bartholomew, Courtenay; Edwards, Jeffrey; Gomez, Perry; Blattner, William A. .
Afiliação
  • Saville, Rebecca D. ; University of Maryland. School of Medicine. Department of Pathology. Maryland. United States of America
  • Constantine, Niel T. ; University of Maryland. School of Medicine. Department of Pathology. Maryland. United States of America
  • Cleghorn, Farley R. ; University of Maryland. Institute of Human Virology. Maryland. United States of America
  • Jack, Noreen; Medical Research Foundation of Trinidad and Tobago. Port of Spain. Trinidad and Tobago
  • Bartholomew, Courtenay; Medical Research Foundation of Trinidad and Tobago. Port of Spain. Trinidad and Tobago
  • Edwards, Jeffrey; Medical Research Foundation of Trinidad and Tobago. Port of Spain. Trinidad and Tobago
  • Gomez, Perry; Princess Margaret Hospital. Nassau. Bahamas
  • Blattner, William A. ; University of Maryland. Institute of Human Virology. Maryland. United States of America
Journal of clinical microbiology ; 39(7): 2518-2524, Jul. 2001. tab, graf
Artigo em Inglês | MedCarib | ID: med-17774
Biblioteca responsável: TT5
Localização: TT5; W1, JO5893M
ABSTRACT
The VIDAS HIV DUO Ultra, a fourth-generation immunoassay under development for the simultaneous detection of human immunodeficiency virus type 1 (HIV-1) p24 antigen and antibodies to HIV-1 and HIV-2, was evaluated. The enzyme-linked fluorescence immunoassay, performed on the automated VIDAS instrument, is claimed to detect early and established HIV infection. The assay was challenged with a total of 2,847 samples that included 74 members of 10 seroconversion panels, 9 p24 antigen-only-reactive members of a panel of group M clades, 503 consecutively collected samples from individuals seeking care in the University of Maryland Medical System, 1,010 samples from U.S. blood donors, 1,141 samples from patients in a high-incidence population in Trinidad, 83 samples from a clinic for sexually transmitted diseases in the Bahamas, 10 confirmed HIV-1 group O samples, and 16 confirmed HIV-2 samples from the Cote d'Ivoire. Reference tests were U.S. Food and Drug Administration-licensed HIV antibody screening, p24 antigen tests, HIV confirmatory assays, and the Roche Diagnostics Amplicor HIV-1 Monitor. The VIDAS HIV DUO Ultra demonstrated 100% sensitivity and 99.5% specificity overall, with a 99.7% specificity in low-risk individuals. The analytical sensitivity, as assessed by seroconversion panels and p24 antigen in samples, was equivalent to the sensitivity of the reference assays used to characterize these panels. The VIDAS HIV DUO Ultra is accurate, offers potential advantages over conventional HIV testing for time and cost savings, has walk-away capability, and correctly identifies both early and established HIV infections.
Assuntos
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MedCarib Assunto principal: Ensaio de Imunoadsorção Enzimática / Anticorpos Anti-HIV / Proteína do Núcleo p24 do HIV Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Limite: Feminino / Humanos / Masculino País/Região como assunto: Caribe Inglês / Trinidad e Tobago Idioma: Inglês Revista: Journal of clinical microbiology Ano de publicação: 2001 Tipo de documento: Artigo Instituição/País de afiliação: Medical Research Foundation of Trinidad and Tobago/Trinidad and Tobago / Princess Margaret Hospital/Bahamas / University of Maryland/United States of America
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MedCarib Assunto principal: Ensaio de Imunoadsorção Enzimática / Anticorpos Anti-HIV / Proteína do Núcleo p24 do HIV Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Limite: Feminino / Humanos / Masculino País/Região como assunto: Caribe Inglês / Trinidad e Tobago Idioma: Inglês Revista: Journal of clinical microbiology Ano de publicação: 2001 Tipo de documento: Artigo Instituição/País de afiliação: Medical Research Foundation of Trinidad and Tobago/Trinidad and Tobago / Princess Margaret Hospital/Bahamas / University of Maryland/United States of America
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