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Serum IgG reactivity with 41-, 31- and 28-kDa larval proteins of Strongyloides stercoralis in individuals with strongyloidiasis
J Infect Dis ; 168(3): 784-7, Sept. 1993.
Artigo em Inglês | MedCarib | ID: med-2120
Biblioteca responsável: JM3.1
Localização: JM3.1; R11.J71
ABSTRACT
Proteins from a deoxycholate-soluble extract of Strongyloides stercoralis infective larvae were separated by SDS-PAGE, blotting onto nitrocellulose paper, and reacted with sera from individuals with confirmed S. stercoralis infections (n = 100), suspectedS. stercoralis infections in whom no larvae could be detected (n = 27), and other nematode infections (40 with Wuchereria bancrofti, 20 with Onchocerca volvulus, 20 with Necator americanus, and 20 with mixed Ascaris lumbricoides and Trichuris trichiura infections). Immunodominant proteins of approximately 41, 31, and 28 kDa were recongnized by IgG in 91 percent, 88 percent and 90 percent respectively, of sera from those with confirmed strongyloidiasis; in 100 percent, 100 percent, and 93 percent of sera from those with suspected strongyloidiasis; and in 9 percent, 12 percent and 14 percent of sera from those infected with other nematodes. IgG reactivity to each of these proteins was a more specific means of immunodiagnosis than the currently use indirect ELISA; the methods were equally sensitive.(AU)
Assuntos
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Coleções: Bases de dados internacionais Contexto em Saúde: Doenças Negligenciadas Problema de saúde: Helmintíase / Doenças Negligenciadas / Oncocercose / Zoonoses Base de dados: MedCarib Assunto principal: Estrongiloidíase / Imunoglobulina G / Anticorpos Anti-Helmínticos / Strongyloides stercoralis / Antígenos de Helmintos Tipo de estudo: Estudo diagnóstico Idioma: Inglês Revista: J Infect Dis Ano de publicação: 1993 Tipo de documento: Artigo
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Coleções: Bases de dados internacionais Contexto em Saúde: Doenças Negligenciadas Problema de saúde: Helmintíase / Doenças Negligenciadas / Oncocercose / Zoonoses Base de dados: MedCarib Assunto principal: Estrongiloidíase / Imunoglobulina G / Anticorpos Anti-Helmínticos / Strongyloides stercoralis / Antígenos de Helmintos Tipo de estudo: Estudo diagnóstico Idioma: Inglês Revista: J Infect Dis Ano de publicação: 1993 Tipo de documento: Artigo
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