Detection of human papilloma virus (hpv) dna in genital biopsy specimens by in situ hybridization with biotinylated probes

ABSTRACT

The presence of human papilloma virus (HPV) nucleotide sequences in paraffin sections of genital biopsies was examined by in-situ hybridization using non-isotopic, random primer biotin labeled probes representing HPV type 16. Biotin labeling of probes was performed using DNA labeling and commercially provided phenotype detection kit. Hybridization was performed under stringent conditions. The hybrids were detected by using Streptavidin and biotinylated alkaline phosphatase and visualized with an enzyme catalyzed chemiluminescent detection reaction. In-situ hybridization with biotinylated probes was a useful technique for the identification of HPV infection. The results were compared with the results obtained with dot blot hybridization probes. The background with biotinylated probes was very low. Using non radioactive robes the localization of hybrids at the cellular level was not better than dot blot labeled probes (AU)