Antimicrobial and anti-tubercular potential of Streptomyces lomondensis SACC 63 isolated from soil samples in Himachal Pradesh, India
J Environ Biol
; 2020 Jan; 41(1): 131-138
Article
| IMSEAR
| ID: sea-214484
ABSTRACT
Aim:
The antimicrobial and anti-tubercular potential of Actinobacteria isolated from soil in Himachal Pradesh, India, was examined in this study.Methodology:
The bioactive metabolites produced from five selected Actinobacterial cultures SACC-63, SACC-75, SACC-76, SACC-77 and SACC-N10 by agar surface fermentation were tested for their antimicrobial and anti-tubercular efficacy in in-vitro. The taxonomy of SACC-63 was identified as Streptomyces lomondensis using 16S-rRNA sequence analyses.Results:
The ethyl acetate extract of strain SACC-63 showed broad spectrum activity against wide range of bacterial and fungal pathogens at 250 µg per disc concentration. In anti-tubercular screening by luciferase reporter phage (LRP) assay, four actinobacterial strains showed more than 65% inhibition against the standard strain Mycobacterium tuberculosis H37Rv and multi drug resistant (MDR) M. tuberculosis strain at 100 µg ml-1 and 500 µg ml-1 concentrations, except the strain SACC-N10. The MIC values of SACC-63 against microbial pathogens ranged between 62.5 µg ml-1 and 250 µg ml-1. The strain SACC-63 showed 99% similarity with Streptomyces spp. Further, phylogenetic analysis based on 16S rRNA revealed a close relationship between SACC-63 and S. lomondensis.Interpretation:
The present study suggested that S. lomondensis SACC-63 isolated from Himachal Pradesh region in India could be a promising source for the production of bioactive molecules.
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Base de dados:
IMSEAR
Revista:
J Environ Biol
Ano de publicação:
2020
Tipo de documento:
Article