The Effect of Human Umbilical Cord Blood Derived Mesenchymal Stem Cell Therapy in Rat Model of Cavernosal Nerve Injury / 대한남성과학회지
Korean Journal of Andrology
; : 136-141, 2008.
Article
em Ko
| WPRIM
| ID: wpr-134460
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: To find out the effect of transplanted umbilical cord blood derived human mesenchymal stem cells (hUCB- MSCs) in the rat model of bilateral cavernosal nerve injury (BCNX) MATERIALS AND METHODS: Male Sprague Dawley rats were divided into three groups: Group I (7 rats, Control, Sham operation) had only bilateral cavernosal nerve crushing injury. PKH-26 fluorescent cell linker was labeled to hUCB- MSCs Group II (7 rats) had transplantation of hUCB- MSCs (3x10(5)cells/50microliter PBS) into MPG after BCNX. Group III (7 rats) had transplantation of hUCB- MSCs (3x10(5)cells/50microliter PBS) into the CC after BCNX. We detected the PKH-26 labeled MSCs in Group II, and III at four weeks after transplantation. Peak intracavernosal pressure (ICP) and ICP/mean arterial pressure (MAP) were recorded after pelvic nerve stimulation at 12 weeks after transplantation. RESULTS: PKH-26 labeled hUCB-MSCs were detected in MPG and CC of group II and III at four weeks after transplantation. The peak ICP was significantly higher in the Group II (69.6+/-5.9mmHg) and Group III (59.7+/-7.6mmHg) compared to Group I40.6+/-10.5mmHg) at 12 weeks after transplantation. The rates of ICP/MAP were significantly higher in Group II (32.2+/-6.4%) and Group III (57.8+/-4.9%) compared to Group I (49.5+/-2.9%) at 12 weeks after transplantation. CONCLUSIONS: The intracavernosal and major pelvic ganglion transplantation of hUCB- MSCs improve erectile function in a rat model of neurogenic impotence.
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Base de dados:
WPRIM
Assunto principal:
Compostos Orgânicos
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Cordão Umbilical
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Salicilamidas
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Ratos Sprague-Dawley
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Transplantes
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Cistos Glanglionares
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Sangue Fetal
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Células-Tronco Mesenquimais
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Pressão Arterial
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Compressão Nervosa
Limite:
Animals
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Humans
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Male
Idioma:
Ko
Revista:
Korean Journal of Andrology
Ano de publicação:
2008
Tipo de documento:
Article