Effects of CGRP on LPS-induced MMP-9 secretion by alveolar macrophages / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 211-215, 2007.
Article
em Zh
| WPRIM
| ID: wpr-253443
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To explore the effects of calcitonin-gene-related peptide (CGRP) on LPS-induced MMP-9 secretion by alveolar macrophages (AM) in vitro.</p><p><b>METHODS</b>The supernatant of LPS-induced Wistar rat AM from different intervention groups were collected to measure the activity by gelatin zymography.</p><p><b>RESULTS</b>(Only secreting a small amount of MMP-9 with unstimulated AM, LPS stimulated MMP-9 production in a concentration-dependent manner (p < 0.01). (2) The activity of MMP-9 in CGRP intervention groups at different levels were significantly lower than those in non-intervention group (p < 0.01). (3) The inhibiting effects of CGRP were diminished by H-7 and W-7, an antagonist of protein kinase C (PKC) and calmodulin (CaM) (p < 0.05).</p><p><b>CONCLUSION</b>These data suggested that CGRP involved in the MMP-9 secretion by AM, partly, via PKC and CaM pathway.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Células Cultivadas
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Lipopolissacarídeos
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Macrófagos Alveolares
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Ratos Wistar
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Receptores de Peptídeo Relacionado com o Gene de Calcitonina
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Secreções Corporais
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Metaloproteinase 9 da Matriz
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Metabolismo
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2007
Tipo de documento:
Article