Comparative study of human fetal and adult bone marrow derived mesenchymal stem cells / 中华血液学杂志
Chinese Journal of Hematology
; (12): 645-648, 2002.
Article
em Zh
| WPRIM
| ID: wpr-261370
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the differences of phenotype and biological characteristics between fetal and adult bone marrow derived mesenchymal stem cells.</p><p><b>METHODS</b>Mononuclear cells from 4-5 months old human aborted fetus and normal adult bone marrow were cultured in SF medium to obtain mesenchymal stem cells. The growth curve, cell cycle, immunophenotype, in vitro expansion potential, differentiation capacities were investigated.</p><p><b>RESULTS</b>The adherent fetal and adult bone marrow-derived cells cultured in the absence of differentiation stimuli gave rise to a population of cells with phenotypical features of mesenchymal stem cells (MSC). These MSCs were similar in cell morphology and antigenic phenotype. The proliferative and multilineage differentiation potential of the bone marrow derived MSC from the fetus is higher than that from the adult, but the adherent ability of the MSCs from the adult is higher than that from the fetus.</p><p><b>CONCLUSION</b>Fetal bone marrow derived MSCs should be enough to sustain a steady supply of low differentiated cells for proliferation, hence an abundant and accessible cellular reservoir for stem cell bioengineering, whereas adult bone marrow derived MSCs are more useful in hematopoietic reconstitution in bone marrow transplantation.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Células da Medula Óssea
/
Microscopia Eletrônica
/
Ciclo Celular
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Diferenciação Celular
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Células Cultivadas
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Imunofenotipagem
/
Linhagem da Célula
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Biologia Celular
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Proliferação de Células
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Alergia e Imunologia
Limite:
Adult
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Ano de publicação:
2002
Tipo de documento:
Article
País de publicação:
CHINA
/
CN
/
REPUBLIC OF CHINA