Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 13-19, 2006.
Article
em En
| WPRIM
| ID: wpr-263230
Biblioteca responsável:
WPRO
ABSTRACT
v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a GST (glutathione S-transferase)-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl beta-D-thiogalactopyranoside (IPTG)-regulated expression, and the fused protein was purified using GSH (glutathione) affinity chromatography. ELISA (enzyme-linked immunosorbent assay) was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression.
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Base de dados:
WPRIM
Assunto principal:
Saccharomyces cerevisiae
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Proteínas de Bactérias
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Proteínas Recombinantes de Fusão
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Engenharia de Proteínas
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Química
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Proteína Oncogênica pp60(v-src)
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Genética
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Glutationa Transferase
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Metabolismo
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Métodos
Idioma:
En
Revista:
Journal of Zhejiang University. Science. B
Ano de publicação:
2006
Tipo de documento:
Article