A probable novel splicing isoform of human vascular endothelial growth factor / 南方医科大学学报
Journal of Southern Medical University
; (12): 755-759, 2012.
Article
em En
| WPRIM
| ID: wpr-269003
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To characterize a new alternative splicing isoform of human vascular endothelial growth factor (VEGF) gene.</p><p><b>METHODS</b>The total RNA was extracted from the lung tissue of a legally aborted 4-month-old fetus and amplified by RT-PCR. The amplified product was cloned into the plasmid pMD18-T and plasmid pcDNA3.1- for sequence analysis.</p><p><b>RESULTS</b>Electrophoresis of the RT-PCR products displayed one short band for VEGF(121) (487 bp) and a long band. The latter was characterized to contain two fragments one was normal VEGF(165) (619 bp), and the other (639 bp) had an identical nucleotide sequence to VEGF(165) with a 20 bp fragment inserted between exons 3 and 4. Sequence analysis showed that this 20-bp nucleotide was inserted from the 3' end of the third intron containing a splicing signal, thus causing shift mutation in the reading frame of VEGF gene and early appearance of the stop codon UAG in the middle of exon 4.</p><p><b>CONCLUSION</b>A new alternative splicing isoform of VEGF probably exists in the lung tissue of a legally aborted human fetus, and its biological significance remains to be further investigated.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Expressão Gênica
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Éxons
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Mutação da Fase de Leitura
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Sequência de Aminoácidos
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Classificação
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Processamento Alternativo
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Isoformas de Proteínas
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Fator A de Crescimento do Endotélio Vascular
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Genética
Limite:
Humans
Idioma:
En
Revista:
Journal of Southern Medical University
Ano de publicação:
2012
Tipo de documento:
Article
País de publicação:
CHINA
/
CN
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REPUBLIC OF CHINA