Cytogenetic differences between adults and children with acute lymphoblastic leukemia: eight-probe fluorescence in situ hybridization and karyotype analyses / 南方医科大学学报
Journal of Southern Medical University
; (12): 707-709, 2012.
Article
em Zh
| WPRIM
| ID: wpr-269016
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the cytogenetic differences between children and adults with acute lymphoblastic leukemia (ALL) using eight-probe fluorescence in situ hybridization and karyotype analysis.</p><p><b>METHODS</b>Eight-probe (MYC, P16, E2A, TEL/AML1, BCR/ABL , MLL , IGH, and hyperdiploidy) fluorescence in situ hybridization and karyotype analysis were performed for 86 adults and 39 children with acute lymphoblastic leukemia.</p><p><b>RESULTS</b>Eight-probe fluorescence in situ hybridization showed significant differences in the positivity rate of TEL/AML1, BCR/ABL, and hyperdiploidy between adult patients and children with ALL. By karyotype analysis, the positivity rate of t(9;22) and hyperdiploidy differed significantly between the children and adult patients (P<0.05).</p><p><b>CONCLUSION</b>Adults and children with ALL have different expression profiles of the fusion genes. Eight-probe fluorescence in situ hybridization is time-saving, accurate and efficient in detecting common genetic abnormalities in ALL patients, and can be well complementary to karyotype analysis in clinical diagnosis of ALL.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Hibridização in Situ Fluorescente
/
Citogenética
/
Diagnóstico
/
Leucemia-Linfoma Linfoblástico de Células Precursoras
/
Cariótipo
/
Genética
/
Cariotipagem
/
Métodos
Tipo de estudo:
Diagnostic_studies
Limite:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2012
Tipo de documento:
Article