Detection of BRAF V600E mutation in hairy cell leukemia by high- resolution melting analysis / 中华血液学杂志
Chinese Journal of Hematology
; (12): 207-209, 2014.
Article
em Zh
| WPRIM
| ID: wpr-295674
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility and diagnostic implication of BRAF V600E mutation identified by high-resolution melting (HRM) assay in patients with hairy cell leukemia (HCL).</p><p><b>METHODS</b>The V600E mutation of BRAF exon 15 in four HCL patients were detected by HRM assay and patients' clinical data were retrospectively analyzed.</p><p><b>RESULTS</b>All four HCL patients were positive for the BRAF V600E mutation, which were identical to the results of DNA sequencing.</p><p><b>CONCLUSION</b>The HRM assay for BRAF V600E mutation provides a useful tool to aid the laboratory diagnosis of HCL with easy operability, accuracy, and low cost.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Análise Mutacional de DNA
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Leucemia de Células Pilosas
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Reação em Cadeia da Polimerase
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Proteínas Proto-Oncogênicas B-raf
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Diagnóstico
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Genética
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Métodos
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Mutação
Tipo de estudo:
Diagnostic_studies
Limite:
Adult
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Aged
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Ano de publicação:
2014
Tipo de documento:
Article