Reversal of apoptotic resistance by Lycium barbarum glycopeptide 3 in aged T cells / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 212-217, 2008.
Article
em En
| WPRIM
| ID: wpr-296062
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study whether Lycium barbarum glycopeptide 3 (LBGP3) affects T cell apoptosis in aged mice.</p><p><b>METHODS</b>LBGP3 was purified with DEAE cellulose and Sephadex columns. Apoptotic "sub-G1 peak" was detected by flow cytometry and DNA ladder was resolved by agarose gel electrophoresis. Levels of IFN-gamma and IL-10 were measured with specific kits and mRNA expression was detected by RT-PCR. Apoptosis-related proteins of FLIP, FasL, and Bcl-2 were determined by Western blotting.</p><p><b>RESULTS</b>LBGP3 was purified from Fructus Lycii water extracts and identified as a 41 kD glycopeptide. Treatment with 200 microg/mL LBGP3 increased the apoptotic rate of T cells from aged mice and showed a similar DNA ladder pattern to that in young T cells. The reversal of apoptotic resistance was involved in down-regulating the expression of Bcl-2 and FLIP, and up-regulating the expression of FasL.</p><p><b>CONCLUSION</b>Lycium barbarum glycopeptide 3 reverses apoptotic resistance of aged T cells by modulating the expression of apoptosis-related molecules.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
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Envelhecimento
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RNA Mensageiro
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Glicopeptídeos
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Linfócitos T
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Química
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Interferon gama
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Interleucina-10
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Apoptose
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Proteínas Proto-Oncogênicas c-bcl-2
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2008
Tipo de documento:
Article