Molecular mechanism of granulocytic differentiation of human promyelocytic leukemia HL-60 cells induced by all-trans retinoic acid / 药学学报
Acta Pharmaceutica Sinica
; (12): 22-28, 2004.
Article
em En
| WPRIM
| ID: wpr-301156
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To elucidate the molecular mechanism of granulocytic differentiation of human promyelocytic leukemia HL-60 cells induced by all-trans-retinoic acid (ATRA).</p><p><b>METHODS</b>Flow cytometry was used to determine the cell cycle changes of HL-60 cells upon ATRA treatment. Gene expression profiles of HL-60 cells induced by 1 mumol.L-1 ATRA were obtained by using cDNA microarrays containing 9,984 genes and expressed sequence tags (ESTs).</p><p><b>RESULTS</b>Cell cycle analysis showed that 48%-73% of cells were arrested at G1/G0 phase upon ATRA treatment; cDNA microarray results demonstrated that the expression of genes encoding adhesion molecules, tissue remodeling proteins, transporters and ribosomal proteins were up-regulated in ATRA treated of HL-60 cells. Several genes involved in oxidase activation pathway were also differentially expressed.</p><p><b>CONCLUSION</b>ATRA treatment induced growth arrest and differentiation in HL-60 cells, which is associated with regulation of the oxidase activation pathway and the expression of tissue remodeling proteins.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Patologia
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Farmacologia
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Tretinoína
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Ciclo Celular
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Diferenciação Celular
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Células HL-60
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Análise de Sequência com Séries de Oligonucleotídeos
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Perfilação da Expressão Gênica
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Granulócitos
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Antineoplásicos
Limite:
Humans
Idioma:
En
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2004
Tipo de documento:
Article