Protective effect of qingxin kaiqiao fang containing cerebrospinal fluid on PC12 cell injury induced by Abeta25-35 / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3271-3274, 2012.
Article
em Zh
| WPRIM
| ID: wpr-308602
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the protective effect of Qingxin Kaiqiao Fang containing cerebrospinal fluid on PC12 cell injury induced by Abeta25-35, in order to provide basis for clinical application of the formula.</p><p><b>METHOD</b>Sprague Dawley rats were orally administration with Qingxin Kaiqiao Fang (7.9 g x kg(-1)) twice a day for 3.5 days to prepare Qingxin Kaiqiao Fang containing cerebrospinal fluid. The nerve cell injury model was established by PC12 cells and Abeta25-35 with the concentration of 10 micromol x L(-1). The expressions of Bax, Bcl-2 and Caspase-3 mRNA were detected by immunohistochemical method in the PC12 cells.</p><p><b>RESULT</b>The Qingxin Kaiqiao Fang group showed a significant higher PC12 cell activity than the model group, with decrease in Bax mRNA and Caspase-3 mRNA expressions and increase in Bcl-2 mRNA expression. There was a significant difference from the model group (P < 0.05, P < 0.01).</p><p><b>CONCLUSION</b>Qinxin Kaiqiao Fang shows a significant protective effect on Abeta25-35-induced nerve cell injury.</p>
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Base de dados:
WPRIM
Assunto principal:
Fragmentos de Peptídeos
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Farmacologia
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Sobrevivência Celular
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Líquido Cefalorraquidiano
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Peptídeos beta-Amiloides
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Células PC12
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Ratos Sprague-Dawley
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Apoptose
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Fármacos Neuroprotetores
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Proteínas Proto-Oncogênicas c-bcl-2
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2012
Tipo de documento:
Article