Bcl-2 inhibitor ABT-737 enhances the cisplatin-induced apoptosis in breast cancer T47D cells / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 891-895, 2011.
Article
em Zh
| WPRIM
| ID: wpr-335368
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of ABT-737 combined with cisplatin on apoptosis of breast cancer cell line T47D cells.</p><p><b>METHODS</b>T47D cells cultured in vitro was used for this experiment. Cell proliferation was measured by MTT assay. The expression of apoptosis-related protein was determined by Western blot. Morphological changes of apoptotic cells were observed by fluorescence microscopy. The apoptosis rate was examined by flow cytometry.</p><p><b>RESULTS</b>The MTT assay showed that ABT-737 significantly decreased the IC(50) of cisplatin in T47D cells [(26.00 ± 1.41) µmol/L of single cisplatin vs. (13.00 ± 1.11) µmol/L of combination (ABT-737 + cisplatin)]. As a single agent, ABT-737 did not inhibit the proliferation of T47D cells, but enhanced the inhibitory effect of cisplatin in a dose-dependent manner. The detection of the cleavage of PARP showed that ABT-737 lowered the doses of cisplatin to induce apoptosis and shortened the induction time of apoptosis in T47D cells. Compared with the single use of cisplatin, the combination of ABT-737 and cisplatin accelerated the cleavage of PARP and caspase3, but did not alter the expression levels of Bcl-2, Bcl-X(L), and Bax. Both flow cytometry and fluorescence microscopy showed that ABT-737 combined with cisplatin significantly increased the apoptosis induction in T47D cells (2.3% ± 0.1 % in the control, 30.0% ± 0.8% in the cisplatin alone, and 49.0% ± 0.5% in the cisplatin + ABT-737 groups, P < 0.05).</p><p><b>CONCLUSION</b>The Bcl-2 inhibitor ABT-737 can significantly enhance cisplatin-induced apoptosis in human breast cancer T47D cells in vitro.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Patologia
/
Farmacologia
/
Piperazinas
/
Sulfonamidas
/
Compostos de Bifenilo
/
Neoplasias da Mama
/
Cisplatino
/
Poli(ADP-Ribose) Polimerases
/
Apoptose
/
Proteínas Proto-Oncogênicas c-bcl-2
Limite:
Female
/
Humans
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Ano de publicação:
2011
Tipo de documento:
Article
País de publicação:
CHINA
/
CN
/
REPUBLIC OF CHINA