Studies on population difference of Dendrobium officinale II establishment and optimization of the method of ISSR fingerprinting marker / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 291-294, 2006.
Article
em Zh
| WPRIM
| ID: wpr-350957
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish and optimize ISSR-PCR system of Dendrobiwn officinale according to the ISSR-PCR characters of D. officinale.</p><p><b>METHOD</b>The effects of ISSR-PCRs was examined by selecting primers and designing different concentrations of the factors in the ISSR-PCRs, the reliable ISSR-PCR systems for D. officinale populations researching was established by analyzing the reasons for occurrence of differential bands and optimizing reaction conditions.</p><p><b>RESULT AND CONCLUSION</b>The optimal ISSR-PCR system in D. officinale was reported for the first time, and 25 15327012 microL ISSR-PCR system contained 10 x Taq buffer, 1.5 U Taq DNA polymerase, 1.2-1.8 mmol x L(-1) MgCl2, 80 micromol x L(-1) dNTP, 0.2 micro mol x L(-1) primer and 20 ng template DNA. The appropriate annealing temperature was among 52-60 degrees C. ISSR PCRs were significantly influenced by Taq DNA polymerase, template DNA quantity and annealing temperature, etc. The ISSR-PCR systems, which were established in this paper for studying D. officinale, could provide clear reliable abundant polymorphisms molecular markers and were proved suitable for studying population authentication and population molecular ecology of D. officinale.</p>
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Plantas Medicinais
/
Sequências Repetitivas de Ácido Nucleico
/
Marcadores Genéticos
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Reação em Cadeia da Polimerase
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Reprodutibilidade dos Testes
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Impressões Digitais de DNA
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Primers do DNA
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DNA de Plantas
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Dendrobium
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Genética
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2006
Tipo de documento:
Article